Hajim School of Engineering and Applied Sciences Department of Chemical Engineering

Faculty

David Wu

David Wu

Professor

Office: 313A Gavett Hall
Phone: (585) 275-8499
Fax: (585) 273-1348
j.wu@rochester.edu
Web Site

PhD, Massachusetts Institute of Technology, 1987

Selected Honors & Awards:

Fellow, American Institute for Medical and Biological Engineering (2011)
Fellow, Society for Industrial Microbiology (2009)
Scientific Advisory Committee, New York State Foundation for Science, Technology & Innovation (2007)
Divisional Lecturer, American Society for Microbiology (2002)
Waksman Outstanding Educator Award, Society for Industrial Microbiology (2005)
Fellow, American Academy of Microbiology (1999)
Visiting Professor, International Center of Biotechnology, Osaka University, Japan (1996)
Undergraduate Teaching Awards, University of Rochester (1989, 1991)

Courses:

ChE 464: Biofuels
ChE 469: Biotechnology & Bioengineering

Research Expertise

Biofuels; Systems Biology; Genomics; Transcriptional Network; Biochemical Engineering; Fermentation; Biocatalysis; Bone Marrow and Lymphoid Tissue Engineering; Molecular Biology. 

Research Overview:

We are interested in bridging life science frontiers and engineering principles to address contemporary biological and biotechnological challenges with a focus on two broad areas. (1) Systems biology approach toward biofuels, where we study the structure-function relationship of the cellulosome of Clostridium thermocellum. The elegant and fascinating cellulosmal structure depicts a new mechanistic concept for biocatalysis and supramolecular organization. Engineering high efficiency cellulase molecules will have a significant impact on climate change. We are now studying the transcription network using the-state-of-art next-generation sequencing and proteomic approaches aiming at developing economically feasible cellulosic biofuels/chemicals processes. (2) Bone marrow and lymph node tissue engineering, where we have taken an engineering approach to culture murine and human marrow cells using a packed-bed bioreactor with artificial scaffolding. The cells grow in three-dimension, simulating the marrow structure. In sharp contrast to the traditional flask culture, which produces only two blood cell types, the bioreactor produces all cell types (granulocytes, erythrocytes, monocytes-macrophages, megakaryocytes, and lymphocytes). The bioreactor provides a novel model for marrow transplantation, gene therapy, and immunotherapy. We have extended the work to human lymph node and developed a lymph node model capable of mounting ex vivo immune responses against vaccines such as HIV.

Recent Publications:

Kuzin, I.; Sun, H. L; Moshkani, S.; Mantalaris, A.; Wu, J.H.D.; Bottaro, A. "Long-term Immunologically Competent Human Peripheral Lymphoid Tissue Cultures in a 3D Bioreactor," Biotechnol. Bioengineering 2011,108, 1430-1440.

Sun, H. L; Tsai, Y. C.; Pietrusz, J.; Nowak, I.; Dertinger, S. D.; Wu, J. H.D.; Chen, Y. C. "Response Kinetics of Radiation-induced Micronucleated Reticulocytes in Human Bone Marrow Culture," Mutat. Res.: Genet. Toxicol. Environ. Mutagen 2010718, 38-43.

Wu, J.H.D.; Newcomb, M.; Sakka, K. "Cohesin-dockerin Interactions and Folding," Bioenergy 2008, 107-113. Washington D.C.: ASM Press.

Newcomb, M.; Chen, C. Y.; Wu, J.H.D. "Induction of the Clostridium Thermocellum CelC Operon by Laminaribiose," Proc. Natl. Acad. Sci. USA2007104, 3747-3752.

Mantalaris, A.; Wu, J.H.D. "Ex vivo Culture of Hematopoietic and Mesenchymal Stem Cells for Tissue Engineering and Cell-Based Therapies," Cell Culture Technology for Pharmaceutical and Cellular Therapies 2006, 723-741. Marcel Dekker, Inc.


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